Jot or JOT may refer to:

Persons with the name Jot Agyeman (born 1967), Ghanaian actor Jot D. Carpenter (1938–2000), American landscape architect Jot Goar (1870–1947), American baseball player

Computing Jot (programming language) The Journal of Object Technology The Joy of Tech, a webcomic Jot, a handwriting recognition system which became the basis for Palm OS' Graffiti 2

Other uses Jot, or Jat, an umbrella term for the peripatetic groups of Afghanistan Jot (interval), a musical pitch interval JOT (TV series), a syndicated, animated television series Jot, a character from The Plucky Squire Jot, the Greek letter iota, in the stock phrase "not one jot or tittle" Joliet Regional Airport (IATA: JOT) Jot, a Dollar Tree private-label brand of office, school, and stationery supplies